新型β-内酰胺类抗生素与氨基糖苷类抗生素联合对铜绿假单胞菌的体外作用

[Combined in vitro effect of new betalactam antibiotics and aminoglycosides against Pseudomonas aeruginosa ].

作者信息

Thabaut A, Durosoir J L, Meyran M

出版信息

Pathol Biol (Paris). 1982 Jun;30(6 Pt 2):549-54.

PMID:6812006

Abstract

The association of 11 betalactam antibiotics (carbenicillin, ticarcillin, azlocillin, piperacillin, mezlocillin, cefotaxim, cefoperazone, ceftriaxon, moxalactam, cefsulodin, ceftazidim) with each of the 3 aminoglycosides (gentamicin, tobramycin, amikacin) were studied by the broth dilution method ("checkboard" technic) against 6 strains of Pseudomonas aeruginosa chosen as a function of their "phenotype". The coefficient of synergy (FIC and FBC) were calculated for the 198 combinations (33 combinations per strain). Bacteriostatic synergy was observed in 18 per cent of the cases and bactericidal synergy in 48 per cent. Synergic association were found to be a function of phenotype. Synergy was rarely observed against bacterial strains resistant to aminoglycosides. Synergy was most frequently observed with two antibiotics in intermediate potency, or an active aminoglycosides combined with an active betalactam antibiotic. The combination containing amikacin produced the most synergy.

摘要

采用肉汤稀释法（“棋盘”技术），研究了11种β-内酰胺类抗生素（羧苄青霉素、替卡西林、阿洛西林、哌拉西林、美洛西林、头孢噻肟、头孢哌酮、头孢曲松、莫拉司亭、头孢磺啶、头孢他啶）与3种氨基糖苷类抗生素（庆大霉素、妥布霉素、阿米卡星）分别组合，对根据“表型”选择的6株铜绿假单胞菌的抗菌作用。计算了198种组合（每种菌株33种组合）的协同系数（FIC和FBC）。在18%的病例中观察到抑菌协同作用，48%的病例中观察到杀菌协同作用。发现协同组合是表型的函数。对氨基糖苷类耐药的菌株很少观察到协同作用。在两种中等效力的抗生素之间，或一种活性氨基糖苷类与一种活性β-内酰胺类抗生素联合使用时，最常观察到协同作用。含阿米卡星的组合产生的协同作用最多。