牛胚泡和子宫内膜对花生四烯酸的体外代谢

Metabolism of arachidonic acid in vitro by bovine blastocysts and endometrium.

作者信息

Lewis G S, Thatcher W W, Bazer F W, Curl J S

出版信息

Biol Reprod. 1982 Sep;27(2):431-9. doi: 10.1095/biolreprod27.2.431.

DOI:10.1095/biolreprod27.2.431

Abstract

Metabolism of arachidonic acid and prostaglandin F2 alpha by bovine blastocysts and endometrial slices recovered on Days 16 and 19 postmating was studied in vitro. In Experiment 1, arachidonic acid (10 microCi tritiated and 200 micrograms radioinert) was added to blastocysts and endometrial slices prior to incubation for 24 h. [3H]arachidonic acid ([3H]AA) and metabolites in extracts of culture medium and tissue homogenates were separated on columns of Sephadex LH-20. Elution profiles of [3H]AA and metabolites in extracts of culture medium revealed that 13, 14-dihydro-15-keto-PGF2 alpha (PGFM), Pge2, PGF2 alpha, and at least four unidentified compounds were produced by Day 16 and Day 19 blastocysts. Endometrial slices from both days of pregnancy produced 3H-prostaglandins. Experiment 2 was conducted to quantify PGE2, PGF2 alpha and PGFM in aliquots of culture medium from Day 16 and Day 19 blastocyst and endometrial incubates. These tissues were incubated with 200 micrograms of radioinert arachidonic acid. Day 16 blastocysts produced less (microgram/blastocyst; P less than 0.01) of each prostaglandin than Day 19 blastocysts (PGE2, 0.7 +/- 0.4 vs. 4.2 +/- 1.0; PGF2 alpha, 2.1 +/- 0.7 vs. 22.8 +/- 4.1; PGFM, 0.03 +/- 0.01 vs. 0.5 +/- 0.2). Endometrial slices produced PGE2, PGF2 alpha and PGFM, but quantities were not affected by day postmating or uterine horncorpus luteum relationships. The third experiment was conducted to determine directly if Day 19 blastocysts and endometrial slices metabolized [3H]PGF2 alpha to [3H]PGFM. Blastocysts and endometrial slices produced [3H]PGFM. Endometrial slices metabolized 34.3 +/- 1.5% of the [3H]PGF2 alpha to [3H]PGFM, while blastocysts metabolized 7.5 +/- 1.6% of the [3H]PGF2 alpha to [3H]PGFM. Results of this study indicate that bovine blastocysts and endometrial slices can metabolize [3H]AA in vitro. It is postulated that prostaglandins of blastocyst and endometrial orgin have a role in maintenance of early pregnancy in cattle.

摘要

研究了在体外对交配后第16天和第19天回收的牛胚泡和子宫内膜切片中花生四烯酸和前列腺素F2α的代谢情况。在实验1中，在孵育24小时之前，将花生四烯酸（10微居里氚标记和200微克非放射性）添加到胚泡和子宫内膜切片中。培养基提取物和组织匀浆中的[3H]花生四烯酸（[3H]AA）及其代谢产物在Sephadex LH - 20柱上进行分离。培养基提取物中[3H]AA及其代谢产物的洗脱图谱显示，第16天和第19天的胚泡产生了13,14 - 二氢 - 15 - 酮 - PGF2α（PGFM）、Pge2、PGF2α以及至少四种未鉴定的化合物。妊娠这两天的子宫内膜切片都产生了3H - 前列腺素。进行实验2以定量第16天和第19天胚泡及子宫内膜培养物的培养基等分试样中的PGE2、PGF2α和PGFM。这些组织与200微克非放射性花生四烯酸一起孵育。第16天的胚泡产生的每种前列腺素（微克/胚泡；P小于0.01）都比第19天的胚泡少（PGE2，0.7±0.4对4.2±1.0；PGF2α；PGE2，0.7±0.4对4.2±1.0；PGF2α，2.1±0.7对22.8±4.1；PGFM，0.03±0.01对0.5±0.2）。子宫内膜切片产生了PGE2、PGF2α和PGFM，但其量不受交配后天数或子宫角/黄体关系的影响。进行第三个实验以直接确定第19天的胚泡和子宫内膜切片是否将[3H]PGF2α代谢为[3H]PGFM。胚泡和子宫内膜切片产生了[3H]PGFM。子宫内膜切片将34.3±1.5%的[3H]PGF2α代谢为[3H]PGFM，而胚泡将7.5±1.6%的[3H]PGF2α代谢为[3H]PGFM。本研究结果表明，牛胚泡和子宫内膜切片在体外能够代谢[3H]AA。据推测，胚泡和子宫内膜来源的前列腺素在维持牛的早期妊娠中起作用。