蛋白质营养不良对衰老过程中真皮胶原蛋白交联的影响。
Effect of protein malnutrition on the crosslinking of dermal collagen in aging.
作者信息
Rao J S, Rao V H, Bose S M
出版信息
Exp Gerontol. 1982;17(3):227-33. doi: 10.1016/0531-5565(82)90029-8.
The effect of protein malnutrition on collagen crosslinking was studied in different age groups of rats. The percent of reversibility of neutral salt-soluble collagen gel and the susceptibility of insoluble collagen to denaturing agents and pronase decreased with aging. The electrophoretic pattern of neutral salt-soluble collagen on sodium dodecyl sulphate polyacrylamide gels showed a decrease of alpha 1 and alpha 2 subunits and an increase of beta-components, resulting in a decrease of alpha/beta ratio in aging. There was a significant decrease in the aldehyde content of neutral salt-soluble collagen during age. Protein deficiency was found to impair the crosslinking and the effect was greater in younger animals than in older ones.
研究了蛋白质营养不良对不同年龄组大鼠胶原蛋白交联的影响。中性盐溶性胶原蛋白凝胶的可逆性百分比以及不溶性胶原蛋白对变性剂和链霉蛋白酶的敏感性随年龄增长而降低。中性盐溶性胶原蛋白在十二烷基硫酸钠聚丙烯酰胺凝胶上的电泳图谱显示，α1和α2亚基减少，β组分增加，导致衰老过程中α/β比值降低。随着年龄增长，中性盐溶性胶原蛋白的醛含量显著下降。发现蛋白质缺乏会损害交联，且对幼龄动物的影响大于老龄动物。
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