Control of endometrial prostaglandin output in vitro during the estrous cycle of the guinea-pig: influence of estradiol 17-beta, progesterone, oxytocin and calcium ionophore A23187.

Agents influencing the output of prostaglandin F2 alpha (PGF2 alpha) from non-pregnant endometrium were investigated by in vitro incubation, for 5 and 19h, using mid-cycle (day 7), end of cycle (day 15), or ovariectomised guinea-pigs. Estradiol 17-beta (10 micrograms/ml) stimulated PGF2 alpha output 24h after incubation with endometrium (p less than 0.05). This stimulation was greater at mid-cycle. Progesterone (50 ng/ml) inhibited output of PGF2 alpha (p less than 0.05) in mid-cycle, end of cycle and ovariectomised guinea-pig cultures. Oxytocin (1 X 10(-5) u/ml) stimulated the output of PGF2 alpha at the end of the cycle, but not at mid-cycle. However, in the presence of estradiol 17-beta (10 micrograms/ml), oxytocin stimulation of mid-cycle PGF2 alpha output was observed. The calcium ionophore A23187 (5 micrograms/ml) stimulated PGF2 alpha synthesis from mid-cycle and end-of-cycle endometrium, and this stimulation resembled that caused by arachidonic acid (100 micrograms/ml), suggesting an action via substrate mobilisation. Co-culture of endometrium and myometrium did not influence endometrial PGF2 alpha or myometrial 6-oxo-PGF2 output. It is suggested that the steroid hormones act as coarse modulators of endometrial PGF2 alpha output, but more rapid changes may be achieved by oxytocin and agents that mobilise substrate supply, possibly via calcium ion fluctuations.