Goldberg I J, Le N A, Ginsberg H N, Paterniti J R, Brown W V
Am J Physiol. 1983 Feb;244(2):E196-201. doi: 10.1152/ajpendo.1983.244.2.E196.
The catabolism of very-low-density lipoprotein apoprotein B and its conversion to low-density lipoprotein was studied in five chow-fed cynomolgus monkeys following injection of radioiodinated homologous very-low-density lipoproteins. The mean (+/- SD) fractional catabolic rate of very-low-density lipoprotein apoprotein B was 0.97 +/- 0.20 h-1 and the mean (+/- SD) production rate was 0.76 +/- 0.20 mg X kg-1 X h-1. The percent of conversion of very-low-density lipoprotein apoprotein B to low-density lipoprotein ranged from 33 to 59%. In separate studies of low-density lipoprotein apoprotein B turnover performed using homologous radiolabeled low-density lipoprotein in five additional animals, the mean (+/- SD) fractional catabolic rate for low-density lipoprotein apoprotein B was 0.050 +/- 0.017 h-1 and the mean (+/- SD) apoprotein B production rate was 0.70 +/- 0.18 mg X kg-1 X h-1. Comparison of the total low-density lipoprotein apoprotein B production with that derived from very-low-density lipoprotein apoprotein B suggested that a large fraction of plasma low-density lipoprotein apoprotein B was derived from a source exclusive of circulating very-low-density lipoprotein apoprotein B. This was confirmed in two animals by simultaneous injection of radiolabeled very-low-density and low-density lipoproteins. Thus, a significant proportion of cynomolgus monkey low-density lipoproteins are produced either by direct hepatic secretion or by rapid conversion of lower-density lipoproteins before they appear in the peripheral circulation.
在五只喂食普通饲料的食蟹猴中，注射放射性碘化的同源极低密度脂蛋白后，研究了极低密度脂蛋白载脂蛋白B的分解代谢及其向低密度脂蛋白的转化。极低密度脂蛋白载脂蛋白B的平均（±标准差）分解代谢率为0.97±0.20 h⁻¹，平均（±标准差）生成率为0.76±0.20 mg·kg⁻¹·h⁻¹。极低密度脂蛋白载脂蛋白B转化为低密度脂蛋白的百分比范围为33%至59%。在另外五只动物中，使用同源放射性标记的低密度脂蛋白对低密度脂蛋白载脂蛋白B的周转进行了单独研究，低密度脂蛋白载脂蛋白B的平均（±标准差）分解代谢率为0.050±0.017 h⁻¹，平均（±标准差）载脂蛋白B生成率为0.70±0.18 mg·kg⁻¹·h⁻¹。将总低密度脂蛋白载脂蛋白B生成量与源自极低密度脂蛋白载脂蛋白B的生成量进行比较，结果表明血浆中很大一部分低密度脂蛋白载脂蛋白B源自不包括循环中的极低密度脂蛋白载脂蛋白B的来源。通过同时注射放射性标记的极低密度脂蛋白和低密度脂蛋白，在两只动物中证实了这一点。因此，相当一部分食蟹猴的低密度脂蛋白是通过肝脏直接分泌或在其出现在外周循环之前由较低密度脂蛋白的快速转化产生的。
