马来酸二乙酯对分离的肝细胞中蛋白质合成的影响。

Effects of diethyl maleate on protein synthesis in isolated hepatocytes.

作者信息

Goethals F, Krack G, Deboyser D, Roberfroid M

出版信息

Toxicology. 1983 Jan;26(1):47-54. doi: 10.1016/0300-483x(83)90055-0.

Abstract

Diethyl maleate is commonly used in toxicological and drug metabolism research using isolated adult rat hepatocytes. At the highest concentrations used the effect of diethyl maleate is, however, not limited to glutathione depletion. In these conditions it inhibits protein synthesis and it impairs the "L" system for amino acid transport. It has, however, no effect on the cytochrome P-450 content or its dependent aldrin monooxygenase. The present report shows that a concentration of diethyl maleate as low as 0.2 mM is sufficient to deplete glutathione without affecting glycogen and protein synthesis, transport of amino acid or monooxygenase activity in isolated adult rat hepatocytes.

摘要

马来酸二乙酯常用于使用成年大鼠分离肝细胞的毒理学和药物代谢研究。然而，在使用的最高浓度下，马来酸二乙酯的作用并不局限于谷胱甘肽耗竭。在这些条件下，它会抑制蛋白质合成，并损害氨基酸转运的“L”系统。然而，它对细胞色素P - 450含量或其依赖的艾氏剂单加氧酶没有影响。本报告表明，低至0.2 mM的马来酸二乙酯浓度足以耗尽谷胱甘肽，而不影响成年大鼠分离肝细胞中的糖原和蛋白质合成、氨基酸转运或单加氧酶活性。