Zhen Y S, Lui M S, Weber G
Cancer Res. 1983 Apr;43(4):1616-9.
Dipyridamole inhibited the incorporation of cytidine, thymidine, uridine, and guanosine in rat hepatoma 3924A cells with 50% inhibitory concentrations of 0.2 to 0.5 microM. For deoxycytidine, the 50% inhibitory concentration was about 100 times higher (23.8 microM). Addition of a combination of cytidine, deoxycytidine, and guanosine, at an optimal concentration of 80 microM each, protected the hepatoma cells from the growth-inhibitory action of the antiglutamine drug, acivicin. The protection provided by the nucleosides was blocked by dipyridamole (6 microM), but not by nitrobenzylthionosine (30 microM). The effect on cell survival of graded concentrations of 0.25 to 1.75 microM acivicin plus dipyridamole (5 microM) and 80 microM concentrations each of cytidine, deoxycytidine, and guanosine was investigated. At an acivicin concentration of 1.75 microM, survivals in the different groups were: (a) acivicin alone, 1%; (b) acivicin plus dipyridamole, 1%; (c) acivicin plus nucleosides, 78%; and (d) acivicin plus nucleosides plus dipyridamole, 3%. Acivicin and dipyridamole were cytotoxic for hepatoma 3924A cells with 50% inhibitory concentrations of 0.5 and 20.3 microM, respectively, as measured by clonogenic assay.
双嘧达莫抑制大鼠肝癌3924A细胞中胞苷、胸苷、尿苷和鸟苷的掺入，其50%抑制浓度为0.2至0.5微摩尔。对于脱氧胞苷，50%抑制浓度约高100倍（23.8微摩尔）。以每种80微摩尔的最佳浓度添加胞苷、脱氧胞苷和鸟苷的组合，可保护肝癌细胞免受抗谷氨酰胺药物阿西维辛的生长抑制作用。核苷提供的保护被双嘧达莫（6微摩尔）阻断，但未被硝苄基硫代肌苷（30微摩尔）阻断。研究了0.25至1.75微摩尔阿西维辛加双嘧达莫（5微摩尔）以及每种80微摩尔胞苷、脱氧胞苷和鸟苷的分级浓度对细胞存活的影响。在阿西维辛浓度为1.75微摩尔时，不同组的存活率为：（a）单独使用阿西维辛，1%；（b）阿西维辛加双嘧达莫，1%；（c）阿西维辛加核苷，78%；（d）阿西维辛加核苷加双嘧达莫，3%。通过克隆形成试验测定，阿西维辛和双嘧达莫对肝癌3924A细胞具有细胞毒性，其50%抑制浓度分别为0.5和20.3微摩尔。
