Gulino A, Pasqualini J R
Endocrinology. 1983 May;112(5):1871-3. doi: 10.1210/endo-112-5-1871.
Estradiol administration to newborn guinea pigs (30 ng/g body wt) provokes an 84 +/- 10% and 145 +/- 10% (means +/- SE) increase of tamoxifen-specific binding site (site B) in the uterine cytosol and in the 0.6 M KCl nuclear extract, respectively, 48 h after hormone administration. The simultaneous injection of progesterone (15 micrograms/g body wt) with estradiol antagonizes this estrogen-induced increase of site B. Parallel modifications of estrogen receptor are observed following the same estradiol treatment: after translocation to the nuclear fraction, cytoplasmic estrogen receptor is replenished 24 h after hormone administration with a net increase of 60 +/- 10% (mean +/- SE) after 48 h. The increase of estrogen receptor is also antagonized by the simultaneous administration of progesterone. It is concluded that the levels of both tamoxifen-specific binding site and estrogen receptor are modulated by estradiol and progesterone in the neonatal uterus of guinea pig.
给新生豚鼠注射雌二醇（30纳克/克体重），在给药48小时后，子宫胞液和0.6M氯化钾核提取物中他莫昔芬特异性结合位点（位点B）分别增加了84±10%和145±10%（平均值±标准误）。同时注射孕酮（15微克/克体重）和雌二醇可拮抗雌激素诱导的位点B增加。在相同的雌二醇处理后，观察到雌激素受体的平行变化：在转移至核部分后，胞质雌激素受体在给药24小时后得到补充，48小时后净增加60±10%（平均值±标准误）。同时注射孕酮也可拮抗雌激素受体的增加。结论是，豚鼠新生子宫中他莫昔芬特异性结合位点和雌激素受体的水平受雌二醇和孕酮调节。
