Synthesis of D-cysteine-related amino acids by 3-chloro-D-alanine chloride-lyase of Pseudomonas putida CR 1-1.

Using the beta-replacement reaction of 3-chloro-D-alanine chloride-lyase from Pseudomonas putida CR 1-1, S-methyl-, S-ethyl-, S-n-propyl-, S-n-butyl-, S-phenyl-, S-benzyl-, S-(2,3-dihydroxypropyl)- and S-(2-hydroxyethyl)-D-cysteine were synthesized from 3-chloro-D-alanine and methyl-, ethyl-, n-propyl-, n-butyl-, phenyl-, benzyl-mercaptans, alpha-thioglycerol and 2-mercaptoethanol, respectively. The enzymatically synthesized D-cysteine-related amino acids were isolated from the large scale reaction mixture and identified physicochemically.