Szibbo C M, Tobe S S
Gen Comp Endocrinol. 1983 Mar;49(3):437-45. doi: 10.1016/0016-6480(83)90207-1.
The control of the decline in C16 juvenile hormone (C16JH) synthesis in the final larval stadium of the viviparous cockroach Diploptera punctata Eschscholtz was investigated to establish how nervous and humoral signals affect the activity of the corpora allata (CA) before metamorphosis. Denervation of the CA in young last instars caused an increase in the rate of JH biosynthesis as monitored by an in vitro radiochemical assay and resulted in a supernumerary larval form at the following ecdysis. However, when larval CA were transplanted into corpus allatectomized adults, they exhibited a further 5- to 10-fold stimulation in synthetic rate and supported host oocyte development at near normal rates. Conversely, adult CA implanted into young and old last instars were inhibited in comparison to those implanted into penultimate instars and adults. These experiments suggest that in D. punctata, an unfavorable humoral environment as well as nervously transmitted inhibitory signals maintain low rates of JH synthesis prior to the imaginal molt.
为了确定神经和体液信号如何在变态前影响咽侧体（CA）的活性，研究了胎生蟑螂双斑扁鳖最后一龄幼虫期C16保幼激素（C16JH）合成下降的控制情况。在最后一龄幼虫早期对CA进行去神经支配，通过体外放射化学分析监测发现，保幼激素生物合成速率增加，并在随后的蜕皮时产生多一个幼虫形态。然而，当将幼虫的CA移植到切除咽侧体的成虫中时，它们的合成速率进一步受到5至10倍的刺激，并以接近正常的速率支持宿主卵母细胞发育。相反，与移植到倒数第二龄幼虫和成虫中的CA相比，移植到最后一龄幼虫早期和晚期的成虫CA受到抑制。这些实验表明，在双斑扁鳖中，不利的体液环境以及神经传递的抑制信号在成虫蜕皮前维持着较低的保幼激素合成速率。
