含离子脂质体对体外灌流的家兔颈动脉体化学传入活动的影响。

Effects of ion-containing liposomes upon the chemoafferent activity of the rabbit carotid body superfused in vitro.

作者信息

Bernon R, Leitner L M, Roumy M, Verna A

出版信息

Neurosci Lett. 1983 Mar 14;35(3):289-95. doi: 10.1016/0304-3940(83)90332-4.

Abstract

The effects of calcium concentration changes in carotid body cells on the chemoreceptor discharges were studied in vitro on carotid bodies removed from anaesthetized rabbits. Addition of calcium-containing liposomes to the superfusing medium increased the chemoreceptors' discharges. This effect was abolished by hyperoxia or when EGTA-containing liposomes were simultaneously added with the calcium-containing liposomes. A histological control with ferritin-enriched liposomes showed that the liposome content was transferred into the cellular elements of the preparation except the nerve endings. Results suggest a relationship between calcium concentration changes in carotid body cells and chemoafferent activity.

摘要

在体外对从麻醉兔身上取下的颈动脉体进行研究，以探讨颈动脉体细胞内钙浓度变化对化学感受器放电的影响。向灌流液中添加含钙脂质体可增加化学感受器的放电。高氧或在添加含钙脂质体的同时添加含乙二醇双四乙酸（EGTA）脂质体可消除这种效应。用富含铁蛋白的脂质体进行组织学对照显示，脂质体内容物被转运到制剂的细胞成分中，但神经末梢除外。结果提示颈动脉体细胞内钙浓度变化与化学传入活动之间存在关联。