Sasaki N, Holdsworth G, Barnhart R L, Srivastava L S, Glueck C J, Kashyap M L, Jackson R L
Atherosclerosis. 1983 Mar;46(3):341-52. doi: 10.1016/0021-9150(83)90183-1.
The effect of isocaloric high and low carbohydrate (Carb) diets on the structure and apoprotein composition of plasma high density lipoproteins (HDL) was assessed in four healthy men. The high Carb diet contained 65% calories as Carb and 15% as fat; the low Carb was 15% and 65%, respectively, with protein fixed at 20% of calories in each case. Cholesterol was 400 mg/day and the P/S ratio of the fat was 0.4. Each diet was sequentially consumed for periods of 3 weeks. At the end of each 3-week study period, plasma HDL2 and HDL3 were isolated by zonal ultracentrifugation and their apoprotein and lipid compositions were determined. Compared to the low Carb diet, the high Carb diet was associated with an increase in the size of HDL2 (116.0 +/- 1.8 vs. 109.1 +/- 1.8 A) and in the content (mean weight % +/- SEM) of apoE (2.81 +/- 0.71 vs. 1.79 +/- 0.49, P less than 0.01) and of apoC-II (1.73 +/- 0.09 vs. 1.11 +/- 0.12, P less than 0.01). HDL2 apoC-III content was not significantly different on the two diets (6.49 +/- 0.50 vs. 7.42 +/- 1.21). On the two diets, HDL3 size and HDL3 apoE content were not significantly changed. HDL3 apoC-II and apoC-III, however, were higher on the high Carb diet, P less than 0.05. The ratio (by weight) of HDL2 apoE/HDL2 apoC-II + C-III increased on the high Carb diet compared to the low Carb diet (0.344 +/- 0.058 vs. 0.228 +/- 0.053, P less than 0.01). We suggest that the increased amount of apolipoprotein E in HDL2 may influence its rate of catabolic clearance and may account for the well-known decrease in plasma HDL-cholesterol in subjects on high Carb diets.
在四名健康男性中评估了等热量的高碳水化合物（Carb）饮食和低碳水化合物饮食对血浆高密度脂蛋白（HDL）结构和载脂蛋白组成的影响。高碳水化合物饮食中碳水化合物提供65%的热量，脂肪提供15%的热量；低碳水化合物饮食中碳水化合物和脂肪分别提供15%和65%的热量，每种情况下蛋白质均固定为热量的20%。胆固醇摄入量为400毫克/天，脂肪的P/S比为0.4。每种饮食依次食用3周。在每个3周研究期结束时，通过区带超速离心法分离血浆HDL2和HDL3，并测定其载脂蛋白和脂质组成。与低碳水化合物饮食相比，高碳水化合物饮食与HDL2大小增加（116.0±1.8对109.1±1.8 Å）、载脂蛋白E含量（平均重量%±标准误）增加（2.81±0.71对1.79±0.49，P<0.01）以及载脂蛋白C-II含量增加（1.73±0.09对1.11±0.12，P<0.01）有关。两种饮食中HDL2载脂蛋白C-III含量无显著差异（6.49±0.50对7.42±1.21）。在两种饮食中，HDL3大小和HDL3载脂蛋白E含量无显著变化。然而，高碳水化合物饮食中HDL3载脂蛋白C-II和载脂蛋白C-III含量较高，P<0.05。与低碳水化合物饮食相比，高碳水化合物饮食中HDL2载脂蛋白E/HDL2载脂蛋白C-II + C-III的比例（按重量）增加（0.344±0.058对0.228±0.053，P<0.01）。我们认为，HDL2中载脂蛋白E含量的增加可能会影响其分解代谢清除率，并可能解释高碳水化合物饮食受试者中众所周知的血浆HDL胆固醇降低现象。
