通过不同方法对从交感脾神经分离出的可溶性蛋白质进行分离。

Separation by different methods of soluble proteins isolated from sympathetic splenic nerves.

作者信息

Balzer H, Husseini H

出版信息

J Chromatogr. 1983 Mar 25;259(1):87-96. doi: 10.1016/s0021-9673(01)87981-4.

Abstract

The soluble proteins of sympathetic splenic nerves were separated by high-performance liquid chromatography (HPLC), sodium dodecyl sulphate (SDS)-HPLC on TSK-GEL-SW columns and SDS-polyacrylamide gel electrophoresis (PAGE). With HPLC the main fractions had apparent molecular weights Mr 150,000, 75,000-85,000, 27,000 and less 5000, with SDS-HPLC the Mr values were 75,000, 13,500 and less 5000 and with SDS-PAGE the Mr were 75,000, 55,000, 45,000 and less 12,500. Of the proteins eluted after electrofocusing, the bands of pH 4.0-5.2 showed a single peak of Mr 75,000 in all separation methods used; in the range pH 5.8-6.5, proteins with Mr 150,000 were found by HPLC, 75,000 by SDS-HPLC and 55,000 and 45,000 by SDS-PAGE. The Mr 150,000 fraction in the range pH 5.8-6.5 showed DOPA decarboxylase (E.C. 4.1.1.28) activity. The results provide new information about the soluble proteins of sympathetic nerve.

摘要

通过高效液相色谱（HPLC）、在TSK - GEL - SW柱上进行十二烷基硫酸钠（SDS）-HPLC以及SDS - 聚丙烯酰胺凝胶电泳（PAGE）对交感脾神经的可溶性蛋白质进行分离。采用HPLC时，主要组分的表观分子量Mr分别为150,000、75,000 - 85,000、27,000和小于5000；采用SDS - HPLC时，Mr值分别为75,000、13,500和小于5000；采用SDS - PAGE时，Mr分别为75,000、55,000、45,000和小于12,500。在等电聚焦后洗脱的蛋白质中，在所有使用的分离方法中，pH 4.0 - 5.2的条带显示出单一的Mr为75,000的峰；在pH 5.8 - 6.5范围内，通过HPLC发现Mr为150,000的蛋白质，通过SDS - HPLC发现Mr为75,000的蛋白质，通过SDS - PAGE发现Mr为55,000和45,000的蛋白质。在pH 5.8 - 6.5范围内Mr为150,000的组分显示出多巴脱羧酶（E.C. 4.1.1.28）活性。这些结果为交感神经的可溶性蛋白质提供了新的信息。