McBlain W A, Toft D O
Biochemistry. 1983 Apr 26;22(9):2262-70. doi: 10.1021/bi00278a032.
Avian oviduct progesterone receptor was treated with the 2',3'-dialdehyde derivative of ATP (oATP) in an attempt to demonstrate the presence of nucleotide binding sites on the receptor. oATP, when added to cytosol, inhibited binding by transformed receptor to ATP-Sepharose, DNA-cellulose, phosphocellulose, or isolated nuclei in an irreversible manner. oATP did not disrupt the steroid-receptor complex, but it did alter the ionic properties of the receptor. This was demonstrated by an increased affinity of receptor for DEAE-cellulose and for hydroxylapatite. oATP mimicked the effect of ATP on progesterone receptor with regard to two properties: it altered the rate of receptor inactivation that occurs in the absence of progesterone, and it promoted receptor conversion from an 8S complex to lower sedimenting forms (4-6 S). The action of oATP on the receptor could be blocked by the addition of pyridoxal 5'-phosphate, which has been shown previously to interact with the progesterone receptor. A partial interference of oATP action was also observed when ATP was added. These results indicate that oATP interacts with the progesterone receptor and may be used as an affinity-labeling agent for receptor characterization.
用ATP的2',3'-二醛衍生物（oATP）处理禽输卵管孕酮受体，以试图证明该受体上存在核苷酸结合位点。当将oATP添加到胞质溶胶中时，它会以不可逆的方式抑制转化后的受体与ATP-琼脂糖、DNA-纤维素、磷酸纤维素或分离的细胞核的结合。oATP不会破坏类固醇-受体复合物，但它确实改变了受体的离子特性。这通过受体对DEAE-纤维素和羟基磷灰石的亲和力增加得到证明。在两个特性方面，oATP模拟了ATP对孕酮受体的作用：它改变了在无孕酮情况下发生的受体失活速率，并且它促进受体从8S复合物转化为沉降系数更低的形式（4-6S）。oATP对受体的作用可以通过添加5'-磷酸吡哆醛来阻断，5'-磷酸吡哆醛先前已被证明可与孕酮受体相互作用。当添加ATP时，也观察到oATP作用的部分干扰。这些结果表明，oATP与孕酮受体相互作用，并且可以用作受体表征的亲和标记剂。
