Houdret N, Lamblin G, Scharfman A, Humbert P, Roussel P
Biochim Biophys Acta. 1983 Jul 5;758(1):24-9. doi: 10.1016/0304-4165(83)90005-3.
High molecular weight bronchial glycoproteins, as nearly native as possible, were treated with either 2-mercaptoethanol or 4-aminophenylmercuric acetate (APMA): analytical electrophoresis revealed that a decrease in molecular weight of glycoproteins coincided with the disappearance of some proteins associated with high molecular weight bronchial glycoproteins. These modifications were not observed if high molecular weight bronchial glycoproteins were incubated with paramethylsulphonyl fluoride and EDTA, two synthetic protease-inhibitors, prior to 2-mercaptoethanol or APMA action. These data suggest that protease-antiprotease complexes are associated with bronchial mucins and that reducing agents or APMA activate proteases.
将尽可能接近天然状态的高分子量支气管糖蛋白用2-巯基乙醇或对氨基苯汞乙酸盐（APMA）处理：分析电泳显示，糖蛋白分子量的降低与一些与高分子量支气管糖蛋白相关的蛋白质的消失同时发生。如果在2-巯基乙醇或APMA作用之前，将高分子量支气管糖蛋白与两种合成蛋白酶抑制剂对甲苯磺酰氟和乙二胺四乙酸一起孵育，则不会观察到这些修饰。这些数据表明蛋白酶-抗蛋白酶复合物与支气管粘蛋白相关，并且还原剂或APMA可激活蛋白酶。
