Kunert K J, Tappel A L
Lipids. 1983 Apr;18(4):271-4. doi: 10.1007/BF02534701.
Measurements of pentane and ethane as indices of in vivo lipid peroxidation were made on samples of breath from vitamin C-sufficient and vitamin C-deficient guinea pigs injected with 23 microliters carbon tetrachloride (CCl4)/100 g body wt. Vitamin C-deficient animals produced significantly more pentane and ethane after CCl4 treatment than did vitamin C-sufficient guinea pigs. Pretreatment of vitamin C-deficient animals with 75 mg ascorbic acid/100 g body wt significantly lowered both pentane and ethane evolution. Protection against in vivo lipid peroxidation similar to that provided by ascorbic acid was also found when vitamin C-deficient guinea pigs were pretreated with isoascorbic acid, reduced glutathione, alpha-tocopherol or beta-carotene. When animals were pretreated with the radical scavenger mannitol, a protective effect was also observed as measured by pentane evolution.
以戊烷和乙烷的含量作为体内脂质过氧化的指标，对注射了23微升四氯化碳（CCl4）/100克体重的维生素C充足和维生素C缺乏的豚鼠的呼吸样本进行了测量。维生素C缺乏的动物在接受CCl4处理后产生的戊烷和乙烷明显多于维生素C充足的豚鼠。用75毫克抗坏血酸/100克体重对维生素C缺乏的动物进行预处理，可显著降低戊烷和乙烷的生成量。当用异抗坏血酸、还原型谷胱甘肽、α-生育酚或β-胡萝卜素对维生素C缺乏的豚鼠进行预处理时，也发现了类似于抗坏血酸提供的对体内脂质过氧化的保护作用。当用自由基清除剂甘露醇对动物进行预处理时，通过戊烷生成量的测量也观察到了保护作用。
