[Use of semipermeable membranes in several histochemical reactions].

A method for using semi-impermeable membranes in histochemical reactions is described by the example of studying the activity of acid phosphatase in white mouse kidneys. The advantage of this method over the classical histochemical methods of detection of the acid phosphatase activity in the kidney is that it does not require fixation of the material to be examined, permits a long-term storage of the preparations necessary for repeated histoenzymatic-chemical studies and is highly accurate in the determination of the enzyme activity because the losses of its activity unavoidable with the use of a liquid incubation medium are absent in this method.