Nogawa H
J Embryol Exp Morphol. 1983 Feb;73:221-32.
Mouse submaxillary epithelium undergoes branching morphogenesis with increase in the curvature of its surface in vivo. Recombination experiments in vitro of the epithelium and mesenchyme between 13- and 14-day rudiments showed (1) that the 14-day mesenchyme more actively induced the epithelium to branch than the 13-day mesenchyme, (2) that the 14-day mesenchyme could produce clefts on smaller epithelial lobes than the 13-day mesenchyme, (3) that the 14-day mesenchyme produced lobes with similar diameter to lobes of the 14-day intact rudiment, and (4) that the lobular morphology of assembled 14-day lobes became obscure in recombinants with the 13-day mesenchyme while it was well maintained in recombinants with the 14-day mesenchyme. From these results it is concluded that the mesenchyme determines the curvature of epithelial surface, and that clefts are formed on the epithelial surface as a result of increase in the epithelial curvature.
在体内，小鼠下颌下腺上皮会经历分支形态发生，其表面曲率增加。对13至14天胚胎的上皮和间充质进行体外重组实验表明：（1）14天的间充质比13天的间充质更能积极诱导上皮分支；（2）14天的间充质比13天的间充质能在更小的上皮叶上产生裂隙；（3）14天的间充质产生的叶直径与14天完整胚胎的叶相似；（4）在与13天间充质的重组体中，组装的14天叶的小叶形态变得模糊，而在与14天间充质的重组体中则能很好地维持。从这些结果可以得出结论，间充质决定上皮表面的曲率，并且上皮表面的裂隙是上皮曲率增加的结果。
