Menard M P, Cousins R J
J Nutr. 1983 Aug;113(8):1653-6. doi: 10.1093/jn/113.8.1653.
Brush border membrane vesicles were used to evaluate the influence of a variety of potential zinc-binding ligands on zinc transport in the rat. Citrate, glutathione (reduced) and picolinate were added to the extravesicular incubation medium at 0.38 mM. Transport was evaluated for up to 60 minutes at an extravesicular zinc concentration of 0.2 mM. Citrate depressed zinc transport at each time point checked. Picolinate depressed transport after 5 minutes of incubation. Glutathione slightly enhanced transport after 60 minutes of incubation. Neither citrate, glutathione nor picolinate had an influence on D-glucose transport. These results suggest that the compounds evaluated do not enhance zinc transport across the brush border surface of the small intestine of the rat.
刷状缘膜囊泡被用于评估多种潜在锌结合配体对大鼠锌转运的影响。柠檬酸盐、谷胱甘肽（还原型）和吡啶甲酸盐以0.38 mM的浓度添加到囊泡外孵育培养基中。在囊泡外锌浓度为0.2 mM的条件下，评估长达60分钟的转运情况。在每个检测的时间点，柠檬酸盐均抑制锌转运。孵育5分钟后，吡啶甲酸盐抑制转运。孵育60分钟后，谷胱甘肽轻微增强转运。柠檬酸盐、谷胱甘肽和吡啶甲酸盐均对D-葡萄糖转运没有影响。这些结果表明，所评估的化合物不会增强锌跨大鼠小肠刷状缘表面的转运。
