Linko K
Transfusion. 1983 Jul-Aug;23(4):310-2. doi: 10.1046/j.1537-2995.1983.23483276864.x.
The effect of centrifugation (5 minutes, 6900 X g) on the amount and particle size of blood microaggregates was studied in order to assess whether centrifugation and filtration by a standard (170 micron) transfusion set filter could be used for aggregate removal instead of microfiltration. Aggregates were quantitated using a method based on nylon screens with graded pore sizes. At the beginning of storage, centrifugation increased the particle size of aggregates only slightly. In 7-day-old blood, aggregates capable of passing a 160 micron screen amounted to 308 +/- 107 (SD) mg of debris protein per liter of blood before and 55 +/- 26 (SD) mg of protein (n = 10) after centrifugation. In 14-day-old blood centrifugation increased the size of aggregates even more effectively: 311 +/- 81 (SD) before and 29 +/- 14 (SD) mg of protein following centrifugation. Microaggregates can thus be removed effectively by the centrifugation and filtration method from blood stored for more than 1 week. A standard transfusion set filter can be used instead of a micropore filter.
为了评估离心（5分钟，6900×g）对血液微聚集体数量和粒径的影响，进而判断离心及通过标准（170微米）输血器过滤器过滤是否可用于去除聚集体而非微滤，对其进行了研究。使用基于具有不同孔径尼龙筛网的方法对聚集体进行定量。在储存开始时，离心仅使聚集体的粒径略有增加。在储存7天的血液中，能够通过160微米筛网的聚集体，离心前每升血液中碎片蛋白含量为308±107（标准差）毫克，离心后为55±26（标准差）毫克蛋白（n = 10）。在储存14天的血液中，离心对聚集体粒径的增大作用更明显：离心前为311±81（标准差），离心后为29±14（标准差）毫克蛋白。因此，通过离心和过滤方法可有效去除储存超过1周血液中的微聚集体。可使用标准输血器过滤器代替微孔过滤器。
