Lam D M, Ayoub G S
Vision Res. 1983;23(4):433-44. doi: 10.1016/0042-6989(83)90090-1.
Neurons in the vertebrate retina are interconnected by a complex network of chemical and electrical synapses which make it difficult to study the intrinsic biochemical and biophysical properties of individual cells. During the past decade, enzymatic methods have been developed for dissociating adult retinas into viable and readily identifiable single cells. Using this preparation, we and other investigators have studied the physiological and biochemical properties of several classes of isolated retinal cells. In particular, we have shown that a type of retinal interneuron, the GABA-ergic horizontal cell of teleost retinas, may be an excellent model system for examining the intrinsic membrane properties as well as the cellular mechanisms regulating GABA uptake, synthesis and release from an identified CNS neuron.
脊椎动物视网膜中的神经元通过复杂的化学和电突触网络相互连接，这使得研究单个细胞的内在生化和生物物理特性变得困难。在过去十年中，已经开发出酶促方法，可将成年视网膜解离成有活力且易于识别的单个细胞。利用这种制备方法，我们和其他研究人员研究了几类分离的视网膜细胞的生理和生化特性。特别是，我们已经表明，一种视网膜中间神经元，即硬骨鱼视网膜中的γ-氨基丁酸能水平细胞，可能是一个极好的模型系统，用于研究内在膜特性以及调节γ-氨基丁酸从一个已确定的中枢神经系统神经元摄取、合成和释放的细胞机制。
