Harding C V, Chylack L T, Susan S R, Lo W K, Bobrowski W F
Invest Ophthalmol Vis Sci. 1983 Sep;24(9):1194-202.
Cooperative Cataract Research Group (CCRG) photographic procedures developed by Chylack have made it possible to localize and analyze specific lens opacities for their ultrastructural and chemical characteristics. One group of human lens opacities has been shown to have a high phosphorus/sulfur ratio (as compared to normal lens fiber cells) and an accumulation of unit membranes. The present paper describes another variety of human lens opacity with the following characteristics: (1) high calcium, low sulfur, undetectable phosphorus, as determined by Energy Dispersive X-ray Analysis (EDXA) of bulk specimens in the scanning electron microscope (SEM), or "thick" sections with the transmission mode of the SEM; (2) spheroidal shape; (3) up to approximately 300 microns in size; and (4) birefringence. Microchemical analysis of these opacities shows that the calcium is in the form of calcium oxalate. These calcium-containing opacities, which have been detected in 14 out of 406 human cataractous lenses, have a characteristic morphology, as seen in the CCRG stereo photographs. Therefore, the presence of these calcium opacities, if not obscured by other kinds of opacities, can be detected with a high degree of accuracy in the fresh lens from the CCRG photographs alone.
由奇拉克开发的合作性白内障研究组（CCRG）摄影程序，使得对特定晶状体混浊进行定位并分析其超微结构和化学特征成为可能。已发现一组人类晶状体混浊具有高磷/硫比（与正常晶状体纤维细胞相比）和单位膜堆积。本文描述了另一种具有以下特征的人类晶状体混浊：（1）通过扫描电子显微镜（SEM）中的能谱色散X射线分析（EDXA）或SEM透射模式下的“厚”切片对大块标本进行测定，其钙含量高、硫含量低、磷含量不可测；（2）呈球形；（3）大小可达约300微米；（4）具有双折射性。对这些混浊的微量化学分析表明，钙以草酸钙的形式存在。在406例人类白内障晶状体中有14例检测到了这些含钙混浊，从CCRG立体照片中可以看出，它们具有特征性形态。因此，如果没有被其他类型的混浊所掩盖，仅通过CCRG照片就能在新鲜晶状体中高度准确地检测到这些钙混浊的存在。
