一种用于尿液和血清样本中总雌激素的均相溶胶颗粒免疫分析方法。

A homogeneous sol particle immunoassay for total oestrogens in urine and serum samples.

作者信息

Leuvering J H, Thal P J, White D D, Schuurs A H

出版信息

J Immunol Methods. 1983 Aug 26;62(2):163-74. doi: 10.1016/0022-1759(83)90243-0.

Abstract

Conjugates prepared by adsorption of antibodies to colloidal gold particles were used in a homogeneous sol particle immunoassay for total oestrogens. The assay is based on inhibition by free oestrogens in the sample of the agglutination of a reaction mixture, consisting of the sample, the gold particle anti-oestriol conjugate and a suitable amount of oestriol-16/17-monosuccinyl-bovine serum albumin. The effect of different assay conditions is discussed. Under optimum conditions for an assay performed at room temperature the measuring range for E3 in buffer was 2-10 ng/ml. After appropriate pretreatment of samples the assay can be used to quantitate the level of total oestrogens in both urine and serum. The within-run coefficient of variation was 6.3% and over-all 6.9%. This method is also suitable for the assay of other haptens.

摘要

通过将抗体吸附到胶体金颗粒上制备的结合物用于总雌激素的均相溶胶颗粒免疫测定。该测定基于样品中游离雌激素对反应混合物凝集的抑制作用，反应混合物由样品、金颗粒抗雌三醇结合物和适量的雌三醇-16/17-单琥珀酰牛血清白蛋白组成。讨论了不同测定条件的影响。在室温下进行测定的最佳条件下，缓冲液中E3的测量范围为2-10 ng/ml。对样品进行适当预处理后，该测定可用于定量尿液和血清中总雌激素的水平。批内变异系数为6.3%，总体为6.9%。该方法也适用于其他半抗原的测定。