腺病毒2型早期区域III mRNA 3'末端的序列

The sequence of 3'-termini of mRNAs from early region III of adenovirus 2.

作者信息

Ahmed C M, Chanda R, Stow N, Zain B S

出版信息

Gene. 1982 Oct;19(3):297-301. doi: 10.1016/0378-1119(82)90019-1.

Abstract

The cloned cDNA sequences corresponding to early region III transcripts (76.6-86.0 map units) of adenovirus 2 were analyzed with a view to determine the structure of their 3'-termini. The clones pE7, pE22 and pE34, which contain a part of or the entire coding sequence for the Mr 19000 glycoprotein, showed microheterogeneity with respect to the poly(A) addition site, with the polyadenylation occurring 17, 26, or 29 nucleotides downstream from the hexanucleotide, 5'-ATTAAA-3'. The clone pE17 which represents a copy of the mRNA ending at 86.0 map units showed the presence of the sequence 5'-AATAAA-3', 16 nucleotides before the poly(A) tail.

摘要

对与腺病毒2早期区域III转录本（76.6 - 86.0图单位）相对应的克隆cDNA序列进行了分析，以确定其3'末端的结构。克隆pE7、pE22和pE34包含Mr 19000糖蛋白的部分或全部编码序列，在多聚腺苷酸化位点表现出微异质性，多聚腺苷酸化发生在六核苷酸5'-ATTAAA-3'下游17、26或29个核苷酸处。代表在86.0图单位处结束的mRNA拷贝的克隆pE17在多聚（A）尾之前16个核苷酸处显示出序列5'-AATAAA-3'的存在。