离子载体抗生素的研究。十五、西洛霉素、离子霉素和醚霉素的单价阳离子选择性离子载体活性。

Studies on the ionophorous antibiotics. XV The monovalent cation selective ionophorous activities of carriomycin, lonomycin and etheromycin.

作者信息

Mitani M, Otake N

出版信息

J Antibiot (Tokyo). 1978 Aug;31(8):750-5. doi: 10.7164/antibiotics.31.750.

DOI:10.7164/antibiotics.31.750

Abstract

The cations selectivity profiles of the carboxylic ionophores, carriomycin, lonomycin and etheromycin, have been investigated by measuring the complexation affinities for metal cations and the cation transport activity through an organic barrier. In a two-phase partition study, carriomycin and lonomycin formed complexes more readily with K+ than with NH4+, Rb+ or Na+, but not with Li+ or Cs+. On the other hand, etheromycin exhibited a great preference for K+ or NH4+ over Na+, Li+ or Rb+, but displayed no binding affinity for Cs+. The alklaine degradation product of lonomycin exhibited a preference for K+ or Na+, but its complexation affinities were much lower than those of the parent compound. Carriomycin, lonomycin and etheromycin efficiently transported K+, Rb+ and Na+ through a CCl4 barrier. But did not carry Ca2+. These antibiotics caused a massive release of K+, Rb+ or Na+, but not of Li+ and Cs+, from mitochondria previously loaded with these cations by valinomycin or monazomycin. Thus, it is concluded that carriomycin, lonomycin and etheromycin are monovalent cation selective ionophores.

摘要

通过测量羧酸离子载体、carriomycin、离子霉素和醚霉素对金属阳离子的络合亲和力以及通过有机屏障的阳离子转运活性，研究了它们的阳离子选择性概况。在两相分配研究中，carriomycin和离子霉素与K⁺形成络合物的能力比与NH₄⁺、Rb⁺或Na⁺更强，但与Li⁺或Cs⁺不形成络合物。另一方面，醚霉素对K⁺或NH₄⁺的偏好远高于Na⁺、Li⁺或Rb⁺，但对Cs⁺没有结合亲和力。离子霉素的碱性降解产物对K⁺或Na⁺有偏好，但其络合亲和力远低于母体化合物。Carriomycin、离子霉素和醚霉素能有效地通过CCl₄屏障转运K⁺、Rb⁺和Na⁺，但不能转运Ca²⁺。这些抗生素导致先前通过缬氨霉素或莫那霉素加载了这些阳离子的线粒体大量释放K⁺、Rb⁺或Na⁺，但不释放Li⁺和Cs⁺。因此，得出结论，carriomycin、离子霉素和醚霉素是单价阳离子选择性离子载体。