使用二硫苏糖醇提高人类含Y精子中F小体的可见性。
Use of dithiothreitol for improved visibility of the F body in human Y-bearing spermatozoa.
作者信息
Hegde U C, Shastry P R, Rao S S
出版信息
J Reprod Fertil. 1978 Jul;53(2):403-5. doi: 10.1530/jrf.0.0530403.
PMID:690991
Abstract
Dithiothreitol was used to decondense the nuclear chromatin before staining human spermatozoa with quinacrine for the F body present in Y-bearing spermatozoa. In unseparated fractions and those enriched in X- or Y-bearing spermatozoa, the number of spermatozoa with F bodies increased by 2-9% after dithiothreitol treatment.
摘要
在用喹吖因对人类精子进行染色以检测Y染色体精子中存在的F体之前，使用二硫苏糖醇使核染色质解聚。在未分离的部分以及富含X或Y染色体精子的部分中，经二硫苏糖醇处理后，带有F体的精子数量增加了2% - 9%。
Zotero 插件