Sullivan R, Zuckerman K S, Quesenberry P J
Br J Haematol. 1980 Mar;44(3):365-74. doi: 10.1111/j.1365-2141.1980.tb05905.x.
We studied the effects of high circulating Colony Stimulating Activity (CSA) levels and irradiation induced marrow hypoplasia in CF1 and C57B1/6J host mice upon granulopoiesis in intraperitoneal diffusion chamber (DC) cultures. Serial endotoxin injections resulted in marked elevation of circulating CSA for the first half of an 8 d culture period, and CSA was shown to diffuse into the chamber environment; yet this manipulation alone did not significantly accelerate DC cell growth. Pre-irradiation of the host mice produced no elevation of circulating CSA during the early phase of culture, but resulted in significant stimulation of DC granolopoiesis. Fluctuations in circulating inhibitors of in vitro granulopoiesis did not correlate well with DC cellularity. We conclude that endogenous CSA elevation does not providean effective stimulus per se for granulocyte-monocyte proliferation within DC culture and cannot be solely responsible for mediating the exuberant DC granulopoietic response seen in the pre-irradiated host.
我们研究了CF1和C57B1/6J宿主小鼠体内循环集落刺激活性（CSA）水平升高以及辐射诱导的骨髓发育不全对腹腔扩散盒（DC）培养中粒细胞生成的影响。连续注射内毒素导致在8天培养期的前半段循环CSA显著升高，并且CSA被证明可扩散到盒内环境中；然而，仅这种操作并不能显著加速DC细胞生长。对宿主小鼠进行预照射在培养早期并未使循环CSA升高，但导致DC粒细胞生成受到显著刺激。体外粒细胞生成的循环抑制剂的波动与DC细胞数量的相关性不佳。我们得出结论，内源性CSA升高本身并不能为DC培养中的粒细胞 - 单核细胞增殖提供有效的刺激，并且不能单独介导预照射宿主中所见的旺盛的DC粒细胞生成反应。
