Volsky D J, Ahrlund-Richter L, Dalianis T, Klein G
Eur J Immunol. 1981 Apr;11(4):341-4. doi: 10.1002/eji.1830110415.
Membranes of murine lymphoma cells expressing H-2a antigens were isolated, purified and co-reconstituted with isolated Sendai virus envelopes according to a previously published procedure (Volsky, D.J. et al., Proc. Natl. Acad. Sci. USA 1979. 76: 5440.). The resulting hybrid H-2a/Sendai virus envelope vesicles (SH-2a vesicles) were capable of binding to and fusing with mouse lymphoma cells. The fusion resulted in the implantation of H-2a antigens into membranes of target cells, as demonstrated by the presence of serologically active antigens on cultured cells 8 and 16 h after implantation.
根据先前发表的程序（Volsky, D.J.等人，《美国国家科学院院刊》1979年。76: 5440），分离、纯化表达H-2a抗原的鼠淋巴瘤细胞膜，并与分离的仙台病毒包膜共同重建。所得的杂交H-2a/仙台病毒包膜囊泡（SH-2a囊泡）能够与小鼠淋巴瘤细胞结合并融合。融合导致H-2a抗原植入靶细胞膜，植入后8小时和16小时培养细胞上存在血清学活性抗原证明了这一点。
