Majoor G D, Van de Gaar M J, Van Breda Vriesman P J, Günther E
Immunology. 1981 Jul;43(3):467-73.
Highly purified erythrocytes of inbred LEW (RT1(1)) rats (LEW-E) elicited antibody responses in inbred BN (RT1n) rats. Intravenous infusion of 1 X 10(8) LEW-E elicited IgM haemagglutinins and after one further infusion of LEW-E IgG haemagglutinins were raised as well. These haemagglutinins were mainly directed to RT1.A region-determined classical transplantation antigens but a weak response to non-RT1 minor histocompatibility antigens was also obtained. In the reciprocal combination erythrocytes of BN and LEW.1N rats, which carry the RT1n haplotype on the LEW strain genetic background, infused into LEW rats yielded strong IgM haemagglutinin responses to RT1.A antigens only but failed to elicit IgG antibody. Infusion of LEW.1N rats with LEW-E elicited only weak IgM haemagglutinin responses. These findings demonstrate that RT1.A antigens are immunogenic for they elicit IgM alloantibody responses. IgG haemagglutinin responses to LEW-E may depend on the presence of minor histocompatibility antigens in conjunction with RT1.A antigens on LEW-E or alternatively on control by Ir-genes located outside the RT1 complex.
近交系LEW（RT1(1)）大鼠的高度纯化红细胞（LEW-E）能在近交系BN（RT1n）大鼠中引发抗体反应。静脉输注1×10(8)个LEW-E可引发IgM血凝素，在再次输注LEW-E后，也会产生IgG血凝素。这些血凝素主要针对RT1.A区域决定的经典移植抗原，但对非RT1次要组织相容性抗原也有较弱反应。在相互组合中，携带LEW品系遗传背景上RT1n单倍型的BN和LEW.1N大鼠的红细胞注入LEW大鼠，仅产生对RT1.A抗原的强烈IgM血凝素反应，但未能引发IgG抗体。用LEW-E输注LEW.1N大鼠仅引发较弱的IgM血凝素反应。这些发现表明，RT1.A抗原具有免疫原性，因为它们能引发IgM同种异体抗体反应。对LEW-E的IgG血凝素反应可能取决于LEW-E上与RT1.A抗原结合的次要组织相容性抗原的存在，或者取决于RT1复合体之外的Ir基因的控制。
