一种用于通过小剂量伽马辐射对天然DNA损伤进行体外研究的新电化学方法。

A new electrochemical approach for the in vitro investigation of damage in native DNA by small gamma-radiation doses.

作者信息

Séquaris J M, Valenta P, Nürnberg H W

出版信息

Int J Radiat Biol Relat Stud Phys Chem Med. 1982 Oct;42(4):407-15. doi: 10.1080/09553008214551331.

Abstract

Voltammetry provides a new method to assess gamma-radiation damage to native DNA in vitro after low doses of irradiation. Using single sweep voltammetry at a stationary mercury electrode a substantial labilization of the double helical conformation of gamma-irradiated DNA was found. Up to 40 Gy a linear dose-effect relationship exists reflected for the number of strand breaks and the number of destabilized base pairs. A considerable difference in the frequency of formation of the two kinds of radiation damage is seen in the ratio of their G-values. Base pair labilization occurs depending on aeration conditions 200 to 300 times more frequently than the formation of a single strand break.

摘要

伏安法提供了一种新方法，用于评估低剂量辐照后体外γ射线对天然DNA的损伤。使用固定汞电极上的单扫描伏安法，发现γ辐照DNA的双螺旋构象有显著的不稳定。高达40 Gy时，存在线性剂量效应关系，这反映在链断裂的数量和不稳定碱基对的数量上。两种辐射损伤形成频率的显著差异体现在它们的G值之比中。碱基对的不稳定根据通气条件的不同，其发生频率比单链断裂的形成频率高200至300倍。