Kierszenbaum F, Budzko D B, Miller H C
J Immunol. 1980 Apr;124(4):1599-602.
Treatment of murine spleen cells with normal guinea pig serum selectively abrogated responsiveness of these cells to the T cell mitogens PHA or Con A, but failed to affect responses to LPS, i.e., a B cell-specific mitogen. Although pretreatment with GPS inhibited the in vitro immune response of mouse splenocytes to SRBC, responses were normal after restoration with T cells only, indicating that B cells had been spared by GPS. Consistent with these results, incubation with GPS resulted in the loss of reactivity of mouse lymphoid cells in MLC as well as CML systems, both of which test for T cell activities. Furthermore, parental spleen cells treated with GPS were no longer capable of inducing a GVH reaction in F1 hybrids. When compared, the effects of GPS and anti-Thy-1.2 antibodies plus C were found to be comparable. These results indicate that GPS can selectively remove a number of T cell functions from heterogeneous murine lymphoid cell suspensions. Since spleen macrophages were insensitive to GPS cytotoxicity, lack of T cell function is not likely to be due to depletion of these accessory cells.
用正常豚鼠血清处理小鼠脾细胞可选择性地消除这些细胞对T细胞有丝分裂原PHA或Con A的反应性，但不影响对LPS（即B细胞特异性有丝分裂原）的反应。尽管用GPS预处理会抑制小鼠脾细胞对SRBC的体外免疫反应，但仅用T细胞恢复后反应正常，这表明B细胞未受GPS影响。与这些结果一致，在MLC以及CML系统（两者均检测T细胞活性）中，与GPS孵育导致小鼠淋巴细胞反应性丧失。此外，用GPS处理的亲代脾细胞不再能够在F1杂种中诱导GVH反应。经比较发现，GPS与抗Thy-1.2抗体加补体的作用相当。这些结果表明，GPS可从异质性小鼠淋巴细胞悬液中选择性去除多种T细胞功能。由于脾巨噬细胞对GPS细胞毒性不敏感，T细胞功能的缺失不太可能是由于这些辅助细胞的耗竭所致。
