给予四氢异喹啉后大鼠体内的单胺氧化酶及乙醇代谢酶

Monoamine oxidase and the enzymes of ethanol metabolism in rats after administration of tetrahydroisoquinolines.

作者信息

Ostrovsky Y M, Sadovnik M N, Satanovskaya V I, Omelyanchik M S

出版信息

Adv Exp Med Biol. 1980;126:137-43. doi: 10.1007/978-1-4684-3632-7_13.

Abstract

A mixture of tetrahydroisoquinolines, synthesized by the method of Osswald et al. (1975), was injected intraperitoneally at a dose of 2 mg/kg into rats. After 3 hours, the activity of ethanol-metabolizing enzymes (alcohol dehydrogenase and the microsomal ethanol oxidizing system) and acetaldehyde-metabolizing enzymes (NAD- and NADP-dependent aldehyde dehydrogenases) in liver sub-cellular fractions, as well as the activity of monoamine oxidase with noradrenaline, tyyramine, benzylamine and serotonin as substrates, were investigated in various brain areas. There was observed a decrease in the activity of the microsomal ethanol-oxidizing system and the NAD-dependent mitochondrial aldehyde dehydrogenase with low Km for acetaldehyde, the activity of the other enzymes investigated being invariable. The inhibition of the activity of monoamine oxidase with noradrenaline as a substrate was revealed in the caudate nucleus, cerebellum, truncus cerebri, and cerebral cortex, while only in the cerebral cortex was there revealed an inhibition of monoamine oxidase with the other substrates. The data obtained indicate that tetrahydroisoquinoline alkaloids not only disturb biogenic amine metabolism, but also affect ethanol and acetaldehyde metabolism in animals.

摘要

按照奥斯瓦尔德等人（1975年）的方法合成的四氢异喹啉混合物，以2毫克/千克的剂量腹腔注射到大鼠体内。3小时后，研究了肝亚细胞组分中乙醇代谢酶（乙醇脱氢酶和微粒体乙醇氧化系统）和乙醛代谢酶（NAD和NADP依赖性醛脱氢酶）的活性，以及在不同脑区中以去甲肾上腺素、酪胺、苄胺和5-羟色胺为底物的单胺氧化酶的活性。观察到微粒体乙醇氧化系统和对乙醛具有低Km值的NAD依赖性线粒体醛脱氢酶的活性降低，而所研究的其他酶的活性不变。在尾状核、小脑、脑干部位和大脑皮层中发现以去甲肾上腺素为底物的单胺氧化酶活性受到抑制，而仅在大脑皮层中发现以其他底物为底物的单胺氧化酶受到抑制。所获得的数据表明，四氢异喹啉生物碱不仅会扰乱生物胺代谢，还会影响动物体内的乙醇和乙醛代谢。