Striker G E, Killen P D, Farin F M
Transplant Proc. 1980 Sep;12(3 Suppl 1):88-99.
Human glomerular cells were isolated, identified, and propagated in vitro, and a number of features were found to be unique to glomerular visceral epithelial and mesangial cells. Epithelial cells are identified by their "epithelial" morphology in vitro, growth pattern, presence of C3b surface receptors, response to mitogens, synthesis of a single collagen type (IV), and nearly exclusive synthesis of one type of sulfated glycosaminoglycan, heparan sulfate. Mesangial cells differ from epithelial cells and other potential contaminating cells, such as fibroblasts or endothelial cells, by their characteristic light and electron microscopic morphology and their synthesis of specific collagen and glycosaminoglycan types. In addition, while they closely resemble vascular smooth muscle cells in many ways, they differ with respect to their response to mitogens. It should now be possible to study these cells for other features, including the presence of alloantigens. This property and their response to inflammatory mediators or cells are prerequisites to the determination of their role in the pathogenesis of renal allograft rejection.
人肾小球细胞在体外进行分离、鉴定和增殖，发现肾小球脏层上皮细胞和系膜细胞具有许多独特的特征。上皮细胞通过其体外的“上皮”形态、生长模式、C3b表面受体的存在、对有丝分裂原的反应、单一IV型胶原的合成以及几乎仅合成一种硫酸化糖胺聚糖即硫酸乙酰肝素而得以鉴定。系膜细胞与上皮细胞以及其他潜在的污染细胞，如成纤维细胞或内皮细胞不同，在于其特征性的光镜和电镜形态以及特定类型胶原和糖胺聚糖的合成。此外，虽然它们在许多方面与血管平滑肌细胞非常相似，但在对有丝分裂原的反应方面存在差异。现在应该能够研究这些细胞的其他特征，包括同种异体抗原的存在。这一特性以及它们对炎症介质或细胞的反应是确定它们在肾移植排斥发病机制中作用的先决条件。
