多发性硬化症特异性脑抗原作用诱导白细胞溶酶体中性蛋白酶丧失。
Loss of lysosomal neutral proteinase from leucocytes induced by the action of multiple sclerosis-specific brain antigens.
作者信息
Rastogi S C, Clausen J
出版信息
Clin Exp Immunol. 1980 Oct;42(1):50-6.
Lysosomal neutral proteinase levels were assayed in leukocytes of patients with other neurological diseases before and after the reaction with MS-specific brain antigens (MSG2). The lysosomal neutral proteinase activity of MS leucocytes was found to be higher than in the two other groups under investigation. To assay the effect of MSG2, 1 x 10(6) leucocytes were incubated with 500 ng of protein MSG2 and with 500 ng of protein of a corresponding fraction from control brain (KG2). Intracellular levels of lysosomal neutral proteinase in leucocytes of all the subjects studied were found to be lowered by the action of both MSG2 and KG2. However, loss of intracellular neutral proteinase induced by the action of MSG2 on all leucocytes was significantly higher than that by KG2 (P less than 0 . 0025). It is suggested that macrophages (monocytes) were stimulated to increased lysosomal enzyme activity, initiated by stimulation of T lymphocytes caused by MS-specific brain antigens.
在与多发性硬化症特异性脑抗原（MSG2）反应前后，对患有其他神经系统疾病患者的白细胞中的溶酶体中性蛋白酶水平进行了测定。发现多发性硬化症白细胞的溶酶体中性蛋白酶活性高于所研究的其他两组。为了测定MSG2的作用，将1×10⁶个白细胞与500 ng的蛋白质MSG2以及与来自对照脑相应部分的500 ng蛋白质（KG2）一起孵育。发现所研究的所有受试者白细胞中溶酶体中性蛋白酶的细胞内水平因MSG2和KG2的作用而降低。然而，MSG2对所有白细胞作用所诱导的细胞内中性蛋白酶的损失明显高于KG2（P＜0.0025）。提示巨噬细胞（单核细胞）受到多发性硬化症特异性脑抗原刺激T淋巴细胞所引发的刺激，导致溶酶体酶活性增加。
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