利用荧光抗肌球蛋白和DNA标记来估计原代大鼠心脏细胞培养物中的成肌细胞和心肌细胞数量。

The use of fluorescent antimyosin and DNA labeling for the estimation of the myoblast and myocyte population of primary rat heart cell cultures.

作者信息

Masse M J, Harary I

出版信息

J Cell Physiol. 1981 Jan;106(1):165-72. doi: 10.1002/jcp.1041060117.

Abstract

Cell division in heart muscle cells progressively ceases during the development of the rat heart, leading to an adult stage with muscle cells incapable of cell division. We have quantitatively determined the number of dividing and nondividing heart muscle cells in cultures derived from different stages of the developing rat heart with the use of 3HTdR continuous labeling and fluorescent antimyosin staining. The cultures were derived from 14 and 17 day postcoital (dPC) rat embryos and from 1 and 4 day postnatal (dPN) rats. The percent nondividing cells increased with development and the age of the postnatal rat. The percent nondividing cells in 14 dPC equalled 21%, 17 dPC equalled 25%, 1 dPN equalled 44%, and 4 dPN equalled 60%. This method for the quantitative determination of dividing and nondividing cells in the developing rat heart provides a model that is useful for the study of the mechanism of the loss of cell division capacity.

摘要

在大鼠心脏发育过程中，心肌细胞的细胞分裂逐渐停止，导致成年阶段的心肌细胞无法进行细胞分裂。我们使用³H胸腺嘧啶核苷（³HTdR）连续标记和荧光抗肌球蛋白染色，定量测定了来自发育中大鼠心脏不同阶段的培养物中分裂和不分裂心肌细胞的数量。这些培养物来自交配后14天和17天（dPC）的大鼠胚胎以及出生后1天和4天（dPN）的大鼠。不分裂细胞的百分比随着发育和出生后大鼠的年龄增加而升高。14 dPC时不分裂细胞的百分比为21%，17 dPC时为25%，1 dPN时为44%，4 dPN时为60%。这种定量测定发育中大鼠心脏分裂和不分裂细胞的方法提供了一个模型，可用于研究细胞分裂能力丧失的机制。