生物合成人胰岛素在分离的猪肝细胞上的受体结合
Receptor binding of biosynthetic human insulin on isolated pig hepatocytes.
作者信息
Gammeltoft S
出版信息
Diabetes Care. 1981 Mar-Apr;4(2):235-7. doi: 10.2337/diacare.4.2.235.
PMID:7011734
Abstract
Biosynthetic human insulin (BHI) and pancreatic human insulin were compared with respect to receptor binding in a heterologous assay system: displacement of pork A14-125I-monoiodoinsulin from receptors on pig hepatocytes. The concentrations of human insulin giving half-maximal displacement were identical for both preparations, i.e., 0.5 nM. Their relative potency was 1.01 +/- 0.14 (SD, N = 5), suggesting that biosynthetic and pancreatic human insulin exert the same biologic activity.
摘要
在一个异源检测系统中，对生物合成人胰岛素（BHI）和胰腺人胰岛素的受体结合情况进行了比较：猪A14-125I-单碘胰岛素从猪肝细胞受体上的置换情况。两种制剂使人胰岛素产生半数最大置换的浓度相同，即0.5 nM。它们的相对效价为1.01±0.14（标准差，N = 5），表明生物合成人胰岛素和胰腺人胰岛素具有相同的生物活性。
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