Andreis P G, Armato U
Endocrinology. 1981 May;108(5):1954-64. doi: 10.1210/endo-108-5-1954.
Low doses (10(-14)--10(-11) M) of epidermal growth factor (EGF)/urogastrone and/or equimolar mixtures of glucagon and insulin administered to 4-day-old primary neonatal rat liver cultures stimulated both DNA synthesis and proliferation of hepatocytes within 24 h. The precise roles played in this process by EGF and the two pancreatic hormones were investigated by constructing curves of the fraction of labeled hepatocytes in mitosis and studying the dilution of incorporated [3H]thymidine with time. The results indicate that EGF both activates and then promotes prereplicative development of hepatocytes, while glucagon and insulin only promote prereplicative development.
给4日龄原代新生大鼠肝脏培养物施用低剂量（10^(-14) - 10^(-11) M）的表皮生长因子（EGF）/尿抑胃素和/或胰高血糖素与胰岛素的等摩尔混合物，在24小时内刺激了肝细胞的DNA合成和增殖。通过构建有丝分裂中标记肝细胞的比例曲线并研究掺入的[3H]胸腺嘧啶随时间的稀释情况，研究了EGF和两种胰腺激素在此过程中所起的精确作用。结果表明，EGF既能激活又能促进肝细胞的复制前发育，而胰高血糖素和胰岛素仅促进复制前发育。
