Gruber H E, Schedl H P, Osborne J W
Acta Diabetol Lat. 1980 Jul-Dec;17(3-4):213-23. doi: 10.1007/BF02581321.
The response of islet cells to streptozotocin-induced diabetes was investigated by transmission electron microscopy. Rats were given streptozotocin i.p. (100 mg/kg followed by another dose of 25 mg/kg 24 h later) and sacrificed after 10 days. Pancreata from rats with high serum glucose values (381-553 mg/100 ml) were selected for ultrastructural examination. Islets in these animals were smaller and much less frequent than those of control animals. Cell types were not always easily identifiable in diabetic animals: distinction between A and B cells was often difficult. Cells of some islets displayed diminished electron density and less structured cytoplasm, yet nuclei were not pyknotic. Such cells were usually deficient in secretion granules. A cells were least affected. Cells in the centers of some islets possessed multivesicular bodies, prominent dilated Golgi cisternae and secretory granules which were often pleomorphic. Nuclear envelopes of some peripheral islet cells were swollen and distended; mitochondria were enlarged and the rough endoplasmic reticulum prominent. Some islet cells retained features of B cells in spite of marked ultrastructural alterations. This diversity of morphological findings contrasts with the homogeneity of the population of streptozotocin-diabetic rats with respect to metabolic responses.
通过透射电子显微镜研究胰岛细胞对链脲佐菌素诱导的糖尿病的反应。给大鼠腹腔注射链脲佐菌素（100mg/kg，24小时后再注射25mg/kg），10天后处死。选择血清葡萄糖值高（381 - 553mg/100ml）的大鼠胰腺进行超微结构检查。这些动物的胰岛比对照动物的胰岛小且数量少得多。在糖尿病动物中细胞类型并不总是容易识别：A细胞和B细胞之间的区分常常困难。一些胰岛的细胞显示电子密度降低且细胞质结构较少，但细胞核不是固缩的。这样的细胞通常缺乏分泌颗粒。A细胞受影响最小。一些胰岛中心的细胞具有多囊泡体、明显扩张的高尔基体池和常常形态多样的分泌颗粒。一些周边胰岛细胞的核膜肿胀；线粒体增大且粗面内质网突出。尽管有明显的超微结构改变，一些胰岛细胞仍保留B细胞的特征。这种形态学发现的多样性与链脲佐菌素诱导的糖尿病大鼠群体在代谢反应方面的同质性形成对比。
