Murata K, Tani K, Kato J, Chibata I
J Gen Microbiol. 1980 Oct;120(2):545-7. doi: 10.1099/00221287-120-2-545.
A methylglyoxal-resistant mutant of Escherichia coli B excreted glutathione into the growth medium, especially during growth on medium containing methylglyoxal. In the presence of methylglyoxal, the total amount of glutathione excreted was increased about 50-fold over that of the wild-type strain. The resistant mutant had high activities of two enzyme systems: a glutathione-forming enzyme system (consisting of gamma-glutamylcysteine synthetase and glutathione synthetase) and a glyoxalase system (consisting of glyoxalase I and glyoxalase II). Methylglyoxal resistance appeared to be due to the simultaneous increase in the activities of these two enzyme systems.
大肠杆菌B的一种甲基乙二醛抗性突变体将谷胱甘肽分泌到生长培养基中，尤其是在含有甲基乙二醛的培养基上生长时。在甲基乙二醛存在的情况下，分泌的谷胱甘肽总量比野生型菌株增加了约50倍。该抗性突变体具有两种酶系统的高活性：一种是谷胱甘肽形成酶系统（由γ-谷氨酰半胱氨酸合成酶和谷胱甘肽合成酶组成），另一种是乙二醛酶系统（由乙二醛酶I和乙二醛酶II组成）。甲基乙二醛抗性似乎是由于这两种酶系统活性的同时增加。
