Subramonia Pillai P, Muthukkaruppan V R
Immunology. 1981 Apr;42(4):541-8.
Our previous studies in the lizard, Calotes versicolor showed that low doses of sheep erythrocytes (SRBC) induced bimodal rosette-forming cell (RFC) response, the first RFC peak appearing on the 2nd day and the second on the 10th day after immunization. In the present study, the type of lymphocytes involved in rosette formation has been analysed with reference to their role in the immune response to sheep erythrocytes. One month after adult thymectomy the formation of the early but not the late rosettes was suppressed. The early RFC were sensitive to the in vitro blocking and cytotoxic activity of rabbit anti-lizard thymocyte globulin (ATG), but not the late rosettes. The interrelationship between these two populations has been studied using cyclophosphamide (Cy) and anti-SRBC antiserum. The development of the early RFC was suppressed when Cy was injected with antigen. However, late RFC (B-RFC) were not detected when the drug treatment was postponed to the 7th day of immunization. The absence of any one RFC peak resulted in the inhibition of plaque-forming cell (PFC) response. Thus, the development of early RFC and late RFC occurs independently, although both are required for effective antibody production. The anti-SRBC antiserum inhibited the formation of RFC and PFC responses, when injected along with low doses of SRBC. When antiserum was given 3 days after immunization, both RFC responses occurred to the normal level, even though PFC response was suppressed, however, when the treatment was delayed up to 6 days normal PFC response occurred. Thus, the presence of antigen in the system for up to 6 days is needed for antibody response. The results indicate the sequential appearance to T and B antigen-binding cells in the spleen of the lizard and their interrelationship in antibody production to sheep erythrocytes.
我们之前对变色树蜥的研究表明，低剂量的绵羊红细胞（SRBC）可诱导双峰玫瑰花结形成细胞（RFC）反应，首次RFC峰值出现在免疫后的第2天，第二次出现在第10天。在本研究中，已参照淋巴细胞在对绵羊红细胞免疫反应中的作用，分析了参与玫瑰花结形成的淋巴细胞类型。成年胸腺切除术后1个月，早期而非晚期玫瑰花结的形成受到抑制。早期RFC对兔抗蜥蜴胸腺细胞球蛋白（ATG）的体外封闭和细胞毒性活性敏感，但晚期玫瑰花结则不敏感。已使用环磷酰胺（Cy）和抗SRBC抗血清研究了这两个细胞群体之间的相互关系。当Cy与抗原一起注射时，早期RFC的发育受到抑制。然而，当药物治疗推迟到免疫的第7天时，未检测到晚期RFC（B - RFC）。任何一个RFC峰值的缺失都会导致空斑形成细胞（PFC）反应受到抑制。因此，早期RFC和晚期RFC的发育是独立发生的，尽管两者都是有效抗体产生所必需的。当与低剂量的SRBC一起注射时，抗SRBC抗血清会抑制RFC和PFC反应的形成。当免疫后3天给予抗血清时，尽管PFC反应受到抑制，但两种RFC反应都恢复到正常水平；然而，当治疗延迟至6天时，正常的PFC反应出现。因此，抗体反应需要系统中抗原存在长达6天。结果表明蜥蜴脾脏中T和B抗原结合细胞的相继出现以及它们在对绵羊红细胞抗体产生中的相互关系。
