大肠杆菌外膜和内膜在高铁色素运输中的功能。
Functions in outer and inner membranes of Escherichia coli for ferrichrome transport.
作者信息
Wookey P J, Hussein S, Braun V
出版信息
J Bacteriol. 1981 Jun;146(3):1158-61. doi: 10.1128/jb.146.3.1158-1161.1981.
Abstract
Mutants of the fhuA gene of Escherichia coli K-12, which encodes a receptor protein in the outer membrane, took up ferrichrome after exposure to pronase, whereas fhuB mutants remained transport negative. The latter finding supports our previous proposal that fhuB mutants are defective in a function that residues in the cytoplasmic membrane. Cells remained completely viable after treatment with pronase, although they became sensitive to the antibiotic actinomycin.
摘要
大肠杆菌K-12的fhuA基因编码外膜中的一种受体蛋白，该基因的突变体在暴露于链霉蛋白酶后能摄取高铁色素，而fhuB突变体在转运方面仍为阴性。后一发现支持了我们之前的观点，即fhuB突变体在细胞质膜中的一种功能上存在缺陷。用链霉蛋白酶处理后细胞仍完全存活，尽管它们对放线菌素这种抗生素变得敏感。
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