Riches D W, Morris C J, Stanworth D R
Immunology. 1982 Mar;45(3):473-81.
Complement component C3 has been detected on the plasma membranes of mouse peritoneal macrophages by using an immunoperoxidase technique in conjunction with transmission electron microscopy. Evidence that such cell surface-associated C3 might be the trigger for lysosomal enzyme discharge was sought initially by exposing macrophage monolayers to anti-mouse C3 F(ab')2, and later, by treating cells with the antibody fragment before two potent secretagogues (methylamine and zymosan particles). Both methods, however, failed to demonstrate a role for cell surface-associated C3 in the initiation of enzyme secretion.
运用免疫过氧化物酶技术结合透射电子显微镜,已在小鼠腹膜巨噬细胞的质膜上检测到补体成分C3。最初,通过将巨噬细胞单层暴露于抗小鼠C3 F(ab')2来探寻这种细胞表面相关C3可能是溶酶体酶释放触发因素的证据,后来则是在使用两种强效促分泌剂(甲胺和酵母聚糖颗粒)之前用抗体片段处理细胞。然而,这两种方法均未能证明细胞表面相关C3在酶分泌起始过程中发挥作用。