用于临床急症中苯二氮䓬类药物测定的二苯甲酮衍生物高效液相色谱法
High-performance liquid chromatography of benzophenone derivatives for the determination of benzodiazepines in clinical emergencies.
作者信息
Violon C, Pessemier L, Vercruysse A
出版信息
J Chromatogr. 1982 Feb 5;236(1):157-68. doi: 10.1016/s0021-9673(00)82509-1.
PMID:7061668
Abstract
A screening procedure for fifteen 1,4-benzodiazepines in biological material (urine), suitable for clinical toxicology, is described. The benzodiazepines and/or their metabolites are hydrolysed to their benzophenones; after alkalinization, the benzophenones are extracted with chloroform. The extract is evaporated to dryness and the dried residue is dissolved in methanol and analysed by high-performance liquid chromatography on a reversed-phase column at 254 nm with methanol-water (1:1) as eluent.
摘要
本文描述了一种适用于临床毒理学的生物材料（尿液）中15种1,4-苯二氮䓬类药物的筛查程序。苯二氮䓬类药物及其代谢产物被水解为二苯甲酮；碱化后，用氯仿萃取二苯甲酮。萃取液蒸发至干，干燥残渣溶于甲醇，在反相柱上以甲醇-水（1:1）为洗脱剂于254nm波长处进行高效液相色谱分析。
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