Silva M H, Lee R E, Petrakis N L
Toxicol Lett. 1982 Feb;10(2-3):205-8. doi: 10.1016/0378-4274(82)90075-3.
The vital dyes neutral red (NR), methylene blue (MB) and trypan blue (TB) induced microsomal enzymes that metabolize the chemical carcinogens 2-aminoanthracene (2AA), 6-aminochrysene (6AC) and diaminofluorene (DAF). The Ames test was used as a method of assessing the amount of microsomal enzyme activity that occurred in rat breast and liver when treated with NR, MB, and TB. Livers were more readily induced than mammary glands of midpregnant rats. Liver S-9 fraction from rats treated with vital dyes showed an increase in revertant colonies for 2AA, DAF, and 6AC over that from untreated rats whereas breast S-9 fraction from rats treated with vital dyes showed an increase in revertant colonies for 2AA over untreated.
活体染料中性红（NR）、亚甲蓝（MB）和台盼蓝（TB）可诱导微粒体酶，这些酶能代谢化学致癌物2-氨基蒽（2AA）、6-氨基 Chrysene（6AC）和二氨基芴（DAF）。艾姆斯试验被用作一种评估用NR、MB和TB处理时大鼠乳腺和肝脏中发生的微粒体酶活性量的方法。与妊娠中期大鼠的乳腺相比，肝脏更容易被诱导。用活体染料处理的大鼠的肝脏S-9组分显示，与未处理大鼠相比，2AA、DAF和6AC的回复菌落增加，而用活体染料处理的大鼠的乳腺S-9组分显示，与未处理相比，2AA的回复菌落增加。
