[红外光谱法在伤寒沙门氏菌制剂化学成分定量表征中的潜在应用]
[Potential use of infrared spectroscopy for the quantitative characterization of the chemical composition of Salmonella typhi preparations].
作者信息
Zhurko N P, Savranskaia S Ia, Smirnov S G
出版信息
Zh Mikrobiol Epidemiol Immunobiol. 1982 Mar(3):104-7.
PMID:7080745
Abstract
The possibility of using infrared spectroscopy for the evaluation of the chemical composition of S. typhi preparations is shown. The existence of a clear-cut correlation between the optical density of adsorption bands in the region between 1,665 and 1,100 cm-1 and the content of protein, polysaccharide and nucleic acids in the preparations subjected to analysis has been revealed. The character of changes in the infrared spectra of the preparations at different stages of deep cultivation has been studied.
摘要
研究表明了使用红外光谱法评估伤寒沙门氏菌制剂化学成分的可能性。已揭示出在1665至1100厘米-1区域内吸附带的光密度与所分析制剂中蛋白质、多糖和核酸含量之间存在明确的相关性。还研究了制剂在深层培养不同阶段的红外光谱变化特征。
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