Noguchi T, Fong K L, Lai E K, Olson L, McCay P B
Biochem Pharmacol. 1982 Mar 1;31(5):609-14. doi: 10.1016/0006-2952(82)90439-7.
Treatment of rats with carbon tetrachloride (CCl4) resulted in early reproducible losses of either one or two specific polypeptides (depending on the inducing agent with which the animals had been treated) in the molecular weight range of the multiple forms of cytochrome P-450. The loss was correlated with a decrease in total cytochrome P-450 content in the microsome. The results of this study and those in the accompanying report indicate that CCl4 was metabolized by a specific form of cytochrome P-450 (52,000 daltons), which was rapidly destroyed in the process. The early loss of this peptide occurred simultaneously with the previously demonstrated production of highly reactive trichloromethyl radicals (CCl3). This polypeptide, which was shown to disappear from liver microsomes following treatment of rats with CCl4 was demonstrated in the accompanying report to be the form of cytochrome P-450 specifically required for production of the highly reactive trichloromethyl radical in a reconstituted monooxygenase system.
用四氯化碳（CCl₄）处理大鼠，会导致细胞色素P - 450多种形式分子量范围内的一种或两种特定多肽（取决于动物所接受的诱导剂）出现早期可重现的损失。这种损失与微粒体中细胞色素P - 450总含量的降低相关。本研究结果以及随附报告中的结果表明，CCl₄由一种特定形式的细胞色素P - 450（52,000道尔顿）代谢，该细胞色素P - 450在此过程中迅速被破坏。这种肽的早期损失与先前证明的高活性三氯甲基自由基（CCl₃）的产生同时发生。在用CCl₄处理大鼠后，肝脏微粒体中显示消失的这种多肽，在随附报告中被证明是重组单加氧酶系统中产生高活性三氯甲基自由基所特别需要的细胞色素P - 450形式。
