关于酶的不可逆热失活机制以及“不可逆”失活酶的重新激活可能性。 - Suppr | 超能文献

On the mechanism of irreversible thermoinactivation of enzymes and possibilities for reactivation of "irreversibly" inactivated enzymes.

作者信息

Klibanov A M, Mozhaev V V

出版信息

Biochem Biophys Res Commun. 1978 Aug 14;83(3):1012-7. doi: 10.1016/0006-291x(78)91496-1.

DOI:10.1016/0006-291x(78)91496-1

Abstract

摘要

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On the mechanism of irreversible thermoinactivation of enzymes and possibilities for reactivation of "irreversibly" inactivated enzymes.关于酶的不可逆热失活机制以及“不可逆”失活酶的重新激活可能性。

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Reactivation of enzymes irreversibly denatured at elevated temperature. Trypsin and alpha-chymotrypsin covalently immobilized on Sepharose 4B and in polyacrylamide gel.

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