Kiraly M, Dolivo M
Brain Res. 1982 May 20;240(1):43-54. doi: 10.1016/0006-8993(82)90642-4.
The bioelectric activity of the rat superior cervical ganglion (SCG) infected with pseudorabies virus (PRV) was examined in vitro 30-38 h after inoculation. Simultaneous intra- and extracellular recordings on the internal (ICN) and external carotid nerves (ECN) revealed a synchronized spontaneous activity. This synchronization can be ascribed either to the functional organization of the ganglion or to the mechanism of initiation itself. In the infected ganglia two categories of cells were observed: cells displaying abnormal spontaneous discharges, and silent cells whose electrophysiological behavior was similar to control cells. Spontaneously active cells showed intermittent spiking and bursting activity. The discharge pattern was associated with the firing rate of the emitting cell: sporadically active cells emitted single spikes whereas highly active cells fired bursts of action potentials (APs). Long lasting intracellular recordings demonstrated that the cells undergo gradual changes evolving from sporadic on to high activity. Spontaneous APs usually rode on prepotentials similar to the excitatory postsynaptic potentials (EPSPs). A comparative study of spontaneous prepotentials and orthodromically evoked EPSPs in the same cell demonstrated that the spontaneous prepotentials are real synaptic potentials. No pace-maker potentials were observed. The passive and active electrical membrane properties of spontaneously active neurons were not different from those of silent cells or control cells impaled in uninfected ganglia. D-Tubocurarine abolished the spontaneous activity in the whole ganglion. Ortho- and antidromic electrical stimulations of suprathreshold intensity elicited an evoked response in neurons displaying spontaneous activity, followed by a delayed burst whose shape was similar to the spontaneous burst of the cell. Stimuli of subthreshold intensities induced this delayed burst independently from the evoked response. We conclude that the spontaneous bioelectrical activity is of presynaptic, but not necessarily of preganglionic origin. The possible existence of a cholinergic intraganglionic pathway revealed by the viral infection is discussed.
在接种伪狂犬病病毒（PRV）后30 - 38小时，对体外培养的大鼠颈上神经节（SCG）的生物电活动进行了检测。同时对颈内神经（ICN）和颈外动脉神经（ECN）进行细胞内和细胞外记录，结果显示存在同步的自发活动。这种同步性可能归因于神经节的功能组织，也可能归因于起始机制本身。在受感染的神经节中观察到两类细胞：一类细胞表现出异常的自发放电，另一类是沉默细胞，其电生理行为与对照细胞相似。自发放电的细胞表现出间歇性的尖峰和爆发性活动。放电模式与发放细胞的放电频率相关：偶尔放电的细胞发出单个尖峰，而高活性细胞则发放动作电位（AP）爆发。长时间的细胞内记录表明，这些细胞经历了从偶尔活动到高活性的逐渐变化。自发动作电位通常叠加在类似于兴奋性突触后电位（EPSP）的预电位上。对同一细胞中自发预电位和顺向诱发的EPSP进行的比较研究表明，自发预电位是真正的突触电位。未观察到起搏电位。自发放电神经元的被动和主动电膜特性与沉默细胞或未感染神经节中刺入的对照细胞并无差异。筒箭毒碱消除了整个神经节的自发活动。阈上强度的顺向和逆向电刺激在表现出自发活动的神经元中引发了诱发反应，随后是一个延迟爆发，其形状与细胞的自发爆发相似。阈下强度的刺激独立于诱发反应诱导了这种延迟爆发。我们得出结论，自发生物电活动起源于突触前，但不一定起源于节前。文中还讨论了病毒感染所揭示的神经节内胆碱能通路可能存在的情况。
