Determination of bradykinin, kallidin and Met-Lys-bradykinin by high performance liquid chromatography.

A high performance liquid chromatography system was developed for the quantitative separation and determination of bradykinin, kallidin and Met-Lys-bradykinin using a reversed-phase column (Nucleosil 5 C8) and an isocratic buffer system. The lower limit of detection is 20 pmol for bradykinin. The intra-variation coefficient of the assay is between 1.8 and 5.7%. The recovery of bradykinin is higher than 96%. Addition of human serum albumin to the assay system in a concentration of 40% lead to a remarkable reduction of the detectable kinin level, which indicates an adsorption of kinins to serum albumin.