Moody-Corbett F, Weldon P R, Cohen M W
J Neurocytol. 1982 Jun;11(3):381-94. doi: 10.1007/BF01257984.
Cholinesterase (ChE), detected histochemically, was found to be localized at many sites of nerve-muscle contact in cultures of spinal cord and muscle cells derived from Xenopus laevis embryos. Such contacts were often characterized by a corresponding localization of acetylcholine receptors and by synaptic ultrastructure, including aggregates of clear vesicles in the nerve fibre and an 80-100 nm wide intercellular cleft. The ChE reaction product was localized in the cleft. When cultures were grown in the presence of curare many of the nerve-contacted muscle cells still exhibited ChE at the sites of contact. It is concluded that ChE accumulates at synaptic contacts in these cultures even in the absence of muscle action potentials and contraction.
通过组织化学方法检测发现，在源自非洲爪蟾胚胎的脊髓和肌肉细胞培养物中，胆碱酯酶（ChE）定位于神经 - 肌肉接触的多个部位。此类接触通常具有乙酰胆碱受体的相应定位以及突触超微结构的特征，包括神经纤维中清亮小泡的聚集以及宽80 - 100纳米的细胞间裂隙。ChE反应产物定位于裂隙中。当培养物在箭毒存在的情况下生长时，许多与神经接触的肌肉细胞在接触部位仍表现出ChE。得出的结论是，即使在没有肌肉动作电位和收缩的情况下，ChE仍会在这些培养物的突触接触部位积累。
